曹英秀

现任职称/职务：副教授/博士生导师
通讯地址：北洋园校区54-E321
电子邮箱：caoyingxiu@tju.edu.cn
办公电话：18630880047

论著专利:

代表性论文

Fang LX, Fan J, Luo SL, Chen YR, Wang CY, Cao YX*, Song H*. Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids. Nat Commun. 2021,12(1):4976.

Zhang JL^#, Cao YX^#, Peng YZ, Jin CC, Bai QY, Zhang RS, Liu D, Yuan YJ*. High production of fatty alcohols in Yarrowia lipolytica by coordination with glycolysis. SCI CHINA Chem. 2019, 62(8):1007-1016.

Cao YX^#, Xiao WH^#, Zhang JL, Xie ZX, Ding MZ, Yuan YJ*. Heterologous biosynthesis and manipulation of alkanes in Escherichia coli. Metab Eng. 2016, 38:19-28. 

Cao YX, Xiao WH, Liu D, Zhang JL, Ding MZ, Yuan YJ*. Cao, Ying-Xiu; Xiao, Wen-Hai; Liu, Duo; Zhang, Jin-Lai; Ding, Ming-Zhu; Yuan, Ying-Jin*. Biosynthesis of odd-chain fatty alcohols in Escherichia coli. Metab Eng. 2015, 29:113-123.  

通讯作者SCI论文  

Chen YR^#, Cheng MJ^#, Feng XR, Niu XL, Song H, Cao YX*. Genome editing by CRISPR/Cas12 recognizing AT-rich PAMs in Shewanella oneidensis MR-1. ACS Synth. Biol., 2022, 11(9):2947-2955. 

Chen YR^#, Cheng MJ, Wang L, Li Y, Fang LX, Cao YX*, Song H*. Highly efficient multiplex base editing: one-shot deactivation of eight genes in Shewanella oneidensis MR-1. Synth. Syst. Biotechnol., 2022. 

Li Y^#, Li YY^#, Chen YR, Cheng MJ, Yu H, Song H*, Cao YX*. Coupling riboflavin de novo biosynthesis and cytochrome expression for improving extracellular electron transfer efficiency in Shewanella oneidensis. Biotechnol Bioeng, 2022, 1–13, doi:10.1002/bit.28172 

Fang, LX^#, Li YY^#, Li Y, Cao YX*, Song H*. Transcriptome Analysis to Identify Crucial Genes for Reinforcing Flavins-Mediated Extracellular Electron Transfer in Shewanella oneidensis. Front Microbiol. 2022;13:852527. 

Chen YR^#, Niu XL^#, Cheng MJ, Wang LX, Sun PX, Song H*, Cao YX*. CRISPR/dCas9-RpoD-mediated simultaneous transcriptional activation and repression in Shewanella oneidensis MR-1. ACS Synth. Biol., 2022, 11(6):2184-2192. 

Chen YR^#, Cheng MJ^#, Song H*, Cao YX*. Type I-F CRISPR-PAIR platform for multi-mode regulation to boost extracellular electron transfer in Shewanella oneidensis. iScience, 2022, 25(6):104491. 

Li MX^#, Zhang JL, Bai QY, Fang LX, Song H, Cao YX*. Non-homologous End Joining-Mediated Insertional Mutagenesis Reveals a Novel Target for Enhancing Fatty Alcohols Production in Yarrowia lipolytica. Front Microbiol, 2022. 

Chen YR^#, Fang LX, Ying X, Cheng MJ, Wang L, Sun PX, Zhang ZY, Shi L, Cao YX*, Song H*. Development of whole genome-scale base editing toolbox to promote efficiency of extracellular electron transfer in Shewanella oneidensis MR-1. Adv. Biol., 2022, Mar;6(3):e2101296. 

Fang LX, Fan J, Luo SL, Chen YR, Wang CY, Cao YX*, Song H*. Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids. Nat Commun. 2021, 12(1):4976. 

Bai QY^#, Cheng S^#, Zhang JL, Li MX, Cao YX*, Yuan YJ. Establishment of genomic library technology mediated by nonhomologous end joining mechanism. SCI CHINA LIFE SCI. 2021 Dec;64(12):2114-2128. 

Zhang JL^#, Bai QY^#, Peng YZ, Fan J, Jin CC, Cao YX*, Yuan YJ. High production of triterpenoids in Yarrowia lipolytica through manipulation of lipid components. Biotechnol Biofuels. 2020, 13: 133. 

Jin CC^#, Zhang JL^#, Song H, Cao YX*. Boosting the biosynthesis of betulinic acid and related triterpenoids in Yarrowia lipolytica via multimodular metabolic engineering. Microb Cell Fact.  2019,18(1):77. 

Zhang JL#, Peng YZ#, Liu D, Liu H, Cao YX*, Li, BZ, Li C, Yuan YJ. Gene repression via multiplex gRNA strategy in Y. Lipolytica. Microb Cell Fact . 2018, 17(1):62-74.   

第一作者SCI论文  

Cao YX , Song MY, Li F, Li CF, Lin X, Chen YR, Chen YY, Xu J, Ding Q, Song H*. A synthetic plasmid toolkit for Shewanella oneidensis MR-1. Front Microbiol. 2019, 10(410). 

Zhang JL^#, Cao YX^#, Peng YZ, Jin CC, Bai QY, Zhang RS, Liu D, Yuan YJ*. High production of fatty alcohols in Yarrowia lipolytica by coordination with glycolysis. SCI CHINA Chem. 2019, 62(8):1007-1016.  

Wang CY^#, Cao YX^#, Wang YP, Sun LM, Song H*. Enhancing surfactin production by using systematic CRISPRi repression to screen amino acid biosynthesis genes in Bacillus subtilis. Microb Cell Fact. 2019, 18(1):90.  

Chen XL^#, Cao YX^#, Li F^#, Tian Y, Song H*. Enzyme-Assisted Microbial Electrosynthesis of Poly(3-hydroxybutyrate) via CO₂ Bioreduction by Engineered Ralstonia eutropha. ACS Catal. 2018, 8(5):4429–4437.  

Cao YX^#, Li XF^#, Li F, Song H*. CRISPRi-sRNA: transcriptional-translational regulation of extracellular electron transfer in Shewanella oneidensis. ACS Synth Biol. 2017, 6(9):1679-1690.  

Wang YY^#, Cao YX^#, Li YX, Jin JY, Li JL, Song H*. Asymmetric biosynthesis of intermediates of anti-HIV drugs. Tetrahedron-asymm. 2017, 28(6):745-757.  

Cao YX^#, Xiao WH^#, Zhang JL, Xie ZX, Ding MZ, Yuan YJ*. Heterologous biosynthesis and manipulation of alkanes in Escherichia coli. Metab Eng. 2016, 38:19-28. 

Cao YX, Xiao WH, Liu D, Zhang JL, Ding MZ, Yuan YJ*. Cao, Ying-Xiu; Xiao, Wen-Hai; Liu, Duo; Zhang, Jin-Lai; Ding, Ming-Zhu; Yuan, Ying-Jin*. Biosynthesis of odd-chain fatty alcohols in Escherichia coli. Metab Eng. 2015, 29:113-123. 

Cao YX, Lu H, Qiao B, Chen Y, Yuan YJ*. Comparison of the secondary metabolites in two scales of cephalosporin C (CPC) fermentation and two different post-treatment processes. J Ind Microbiol Biot. 2013, 40:95-103. 

Cao YX, Qiao B, Lu H, Chen Y, Yuan YJ*. Comparison of the secondary metabolites in Penicillium chrysogenum between pilot and industrial penicillin G fermentations. Appl Microbiol Biotechnol. 2011, 89:1193-202. 

Cao YX, Tian HC, Yao K, Yuan YJ*. Simultaneous saccharification and fermentation of sweet potato powder for the production of ethanol under conditions of very high gravity. Front Chem Sci Eng. 2011, 5(3):318-324.   

 

专利

元英进，曹英秀，肖文海，张金来，丁明珠（2016）基因元件、表达载体及其应用，中国发明专利，专利号：ZL201610340212.3 

Yingjin Yuan，Yingxiu Cao，Wenhai Xiao，Duo Liu，Mingzhu Ding，Zexiong Xie，Jinlai Zhang（2015）Genes and uses thereof, methods for synthesizing odd numbered medium chain aliphatic aldehydes and methods for synthesizing even numbered medium chain aliphatic hydrocarbons. 授权号：US2018/273918 A1 

曹英秀，白秋艳，程帅，张金来（2020）一种基于非同源末端连接机制的解脂耶氏酵母基因组整合的方法，中国发明专利，申请号： 2020110895579  Microb Cell Fact. 2018, 17(1):62-74.