李　锋

现任职称/职务：副教授
通讯地址：天津大学北洋园校区化工学院54E-321，天津大学武清前沿技术研究院303
电子邮箱：messilifeng@163.com;feng.li@tju.edu.cn;
办公电话：

论著专利:

       一、一作或通讯论文：

1.       Feng Li#; Yuanxiu Li#; Yingxiu Cao; Lei Wang; Chengguang Liu; Liang Shi; Hao Song*. Modular engineering to increase intracellular total NAD(H/+) level promotes efficiency of extracellular electron transfer of Shewanella oneidensis [J]. Nature Communications, 2018, 9:3637. (Q1, IF=17.694)

2.       Feng Li‡, Rui Tang‡, Baocai Zhang‡, Chunxiao Qiao, Huan Yu, Qijing Liu, Junqi Zhang, Deguang Wu, Liang Shi, and Hao Song*. Full-cycle biofilm engineering to boost electro-conductivity and power production of Shewanella oneidensis [J]. Research, 2023,6:0081. (Q1, IF=11.036)

3.       Juntao Zhao‡, Feng Li ‡, Shutian Kong, Tao Chen; Hao Song, Zhiwen Wang, Elongated riboflavin-producing Shewanella oneidensis in a hybrid biofilm boosts extracellular electron transfer[J]. Advanced Science, 2023, 2206622. (Q1, IF=17.521)

4.       Qijing Liu, Qinran Ding, Wenliang Xu a,b, Yan Zhang, Baocai Zhang, Huan Yu, Chao Li, Junqi Zhang, Zixuan You, Rui Tang, Deguang Wu, Chao Zhao, Yingxiu Cao, Wenyu Lu, Feng Li*, Hao Song*, Engineering cell-electrode interfacial electron transfer to boost power generation of electroactive biofilm [J]. Nano Energy,2023, 117, 108931. (Q1, IF=17.600)

5.       Junqi Zhang, Feng Li*, Dingyuan Liu, Qijing Liu, Hao Song*, Synthetic biology engineering bi-directional extracellular electron transfer of electroactive microorganisms for energy and chemicals production, Chemical Society Reviews,2024, 53, 1375-1446. (Q1, IF=46.2)

6.       Deguang Wu#, Baocai Zhang#, Sicheng Shi#, Rui Tang#, Jichao Jia, Xiaoguang Si, Yifei, Wang, Xi Sun, Dongguang Xiao*, Feng Li*, Hao Song*, Engineering extracellular electron transfer to promote simultaneous brewing wastewater treatment and chromium reduction, Journal of Hazardous Materials, 2024, 465, 133171. (Q1, IF=13.6)

7.       Changjiang Liu‡, Huan Yu‡, Baocai Zhang, Feng Li*, Hao Song*. Engineering whole-cell microbial biosensors for detecting environmental pollution by synthetic biotechnology [J]. Biotechnology Advances, 2022, 60, 108019. (Q1, IF=17.681)

8.       Enze Zhou‡, Feng Li‡, Dawei Zhang#, Dake Xu*, Hao Song*, Derek R. Lovley*. Direct microbial electron uptake as a mechanism for stainless steel corrosion in aerobic environments[J]. Water Research, 2022, 219,118553. (Q1, IF=13.400)

9.       Junqi Zhang‡, Deguang Wu‡, Yakun Zhao, Dingyuan Liu, Xuewu Guo, Yefu Chen, Cuiying Zhang, Xi Sun, Ju Guo, Dezhi Yuan, Dongguang Xiao, Feng Li*, Hao Song*. Engineering Shewanella oneidensis to efficiently harvest electricity power by co-utilizing glucose and lactate in thin stillage of Chinese liquor industry [J], Science of The Total Environment, 2023,855,158696. (Q1, IF=10.753)

10.    Feng Li, Junqi Zhang, Dingyuan Liu, Huan Yu, Chao Li, Qijing Liu, Zheng Chen, Hao Song*. Engineering extracellular polymer substrates biosynthesis and carbon feltcarbon nanotube hybrid electrode to promote biofilm electroactivity and bioelectricity production [J]. Science of The Total Environment, 2023,904,166955. (Q1, IF=10.753)

11.    Juntao Zhao‡, Feng Li‡, Yingxiu Cao, Xinbo Zhang, Tao Chen, Hao Song, Zhiwen Wang. Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms [J]. Biotechnology Advances, 2020, 107682. (Q1, IF=17.681)

12.    Xiaoli Chen#; Yingxiu Cao#; Feng Li#; Yao Tian; and Hao Song*. Enzyme-assisted microbial electrosynthesis of poly-3-hydroxybutyrate via CO₂ bioreduction by engineered Ralstonia eutropha [J]. ACS Catalysis, 2018, 8, 4429-4437. (Q1, IF=12.35)

13.    Baocai Zhang‡, Sicheng Shi‡, Rui Tang‡, Zixuan You, Shulin Shao, Deguang Wu, Huan Yu, Junqi Zhang, Yingxiu Cao, Feng Li*, Hao Song*. Recent advances in enrichment, isolation, and bio-electrochemical activity evaluation of exoelectrogenic microorganisms[J]. Biotechnology Advances, 2023, 66, 108175. (Q1, IF=17.681)

14.    Zixuan You‡, Jianxun Li‡, Yuxuan Wang, Feng Li*, Hao Song*. Engineering electroactive biofilms by synthetic biology strategies[J]. Biotechnology Advances, 2023, 66, 108170. (Q1, IF=17.681)

15.    Baocai Zhang‡, Shulin Shao‡, Huan Yu‡, Qijing Liu, Sicheng Shi, Qingyuan Wu, Feng Li*, Hao Song*. Engineering Shewanella-reduced graphene oxide aerogel biohybrid to efficiently synthesize Au nanoparticles, 2024,168:1,50-61. Journal of Materials Science & Technology, accepted. (Q1, IF=10.391).

16.    Junqi Zhang#, Zixuan You#, Feng Li*, Hao Song*, Structure, function, assembly, and engineering of conductive protein parts for extracellular electron transfer [J]. Quantitative Biology, 2023,1–16.

17.    Qinran Ding‡, Qijing Liu‡, Yan Zhang, Feng Li*, Hao Song*, Modular engineering strategy to redirect electron flux into electron transfer chain for enhancing extracellular electron transfer in Shewanella oneidensis [J]. ACS Synthetic Biology, 2023, 12, 2, 471-481.

18.    Chi Yang‡, Junqi Zhang‡, Baocai Zhang, Dingyuan Liu, Jichao Jia, Feng Li*, Hao Song*. Engineering Shewanella carassii isolated from activated sludge for enhancing methyl orange degradation and bioelectricity harvest [J], Synthetic and Systems Biotechnology, 2022, 7(3): 918-927.

19.    Feng Li#; Yuanxiu Li#; Liming Sun; Xiaoli Chen; Xingjuan An; Changji Yin; Yingxiu Cao; Hui Wu; and Hao Song*, Modular engineering intracellular NADH regeneration boosts extracellular electron transfer of Shewanella oneidensis MR-1 [J]. ACS Synthetic Biology, 2018, 7 (3): 885-895.

20.    Ziying Gong#; Huan Yu#; Junqi Zhang; Feng Li*; Hao Song*, Bidirectional electron transfer-driven chemicals biosynthesis, Synthetic and Systems Biotechnology, 2020, 5: 304-313.

21.    Huan Yu; Yujun Lu; Fei Lan; Yuxuan Wang; Chaoning Hu; Lingfeng Mao; Deguang Wu; Feng Li*; Hao Song*. Engineering outer membrane vesicles to increase extracellular electron transfer of Shewanella oneidensis. ACS Synthetic Biology, 2023, 12, 1645-1656.

22.    Feng Li, Lei Wang, Chenguang Liu, Deguang Wu, Hao Song*. Engineering exoelectrogens by synthetic biology strategies [J]. Current Opinion in Electrochemistry, 2018, 10: 37-45.

23.    Feng Li, Yuanxiu Li, Liming Sun, Xiaofei Li, Changji Yin, Xingjuan An, Yao Tian, HaoSong. Engineering Shewanella oneidensis enables xylose-fed microbial fuel cell [J]. Biotechnology for Biofuels, 2017, 10 (1):196.

24.    Feng Li‡, Changji Yin‡, Liming Sun, Yuanxiu Li, Xuewu Guo, and Hao Song. Synthetic Klebsiella pneumoniae-Shewanella oneidensis consortium enables glycerol-fed high-performance microbial fuel cells [J]. Biotechnology Journal, 2017, 38:148.

25.    Feng Li, Hao Song. Promoting efficiency of microbial extracellular electron transfer by synthetic biology [J]. Chinese Journal of Biotechnology, 2017, (03): 516-534.

26.    Feng Li‡, Xingjuan An‡, Deguang Wu, Yuanyuan Chen, Jing Xu, Yingxiu Cao, Hao Song*. Engineering microbial consortia for high-performance cellulose hydrolyzates-fed microbial fuel cell [J]. Frontiers in Microbiology, 2019, 10: 409.

27.    Junqi Zhang‡, Zheng Chen‡, Changjiang Liu‡, Jianxun Li, Xingjuan An, Deguang Wu, Xi Sun, Feng Li*, Hao Song*, Construction of an acetate metabolic pathway to enhance electron generation of engineered Shewanella oneidensis, Frontiers in Bioengineering and Biotechnology, 2021, 9:757953.

28.    Feng Li‡, Xingjuan An‡, Deguang Wu, Yuanyuan Chen, Jing Xu, Yingxiu Cao, Hao Song*. Engineering microbial consortia for high-performance cellulose hydrolyzates-fed microbial fuel cell [J]. Frontiers in Microbiology, 2019, 10: 409.

29.    Yefu Chen*^, #, Feng Li#, Jian Guo, Guangxin Liu, Xuewu Guo, Dongguang Xiao. Enhanced ethyl caproate production of Chinese liquor yeast by overexpressing EHT1 with deleted FAA1. Journal of Industrial Microbiology and Biotechnology. 2014, 41(3): 563-572.

30.    李锋, 宋浩. 微生物胞外电子传递效率的合成生物学强化[J]. 生物工程学报, 2017, 33(3):516-534.

31.    陈媛媛,张保财,吴德光,李锋*,宋浩. 产电微生物的筛选方法研究进展[J].生物工程学报, 2020, 36(12): 2719-2731.

32.    徐静,由紫暄,张君奇,陈正,吴德光，李锋*,宋浩. 合成生物学方法改造电活性生物膜研究进展[J]. 化工学报,2020, 71(09): 3950-3962.

33.    赵贞尧#,张保财#,李锋*,宋浩*.产电细胞的合成生物学设计构建，化工学报,2021, 72 (1): 468-482.

34.    刘向#,张君奇#,张保财, 杨池, 李锋*，宋浩*，强化产电微生物与电极间电子传递速率的研究进展，生物工程学报, 2021, 37(2): 361-377.

35.    由紫暄,李锋*,宋浩*.电能细胞的合成生物学设计构建,合成生物学,2022,3 (5): 1031-1059.

36.    王川东#，张君奇#，刘丁源，马媛媛，李锋*，宋浩*，“工程微生物共利用木糖和葡萄糖生产化学品进展”，《化工进展》（accepted）

37.    孙盼星#，李锋#，宋浩*，“微生物细胞工厂合成甲基萘醌研究进展”，《科学通报》2022, 67: 1-13.

二、合作论文：

38.    Jin, Shixuan; Feng, Yiyu; Jia, Jichao; Zhao, Fulai; Wu, Zijie; Long, Peng; Li, Feng; Yu, Huitao; Yang, Chi; Liu, Qijing; Zhang, Baocai; Song, Hao*; Feng, Wei*, Three-dimensional N-doped carbon nanotube/graphene composite aerogel anode to develop high-power microbial fuel cell, Energy & Environmental Materials, 2023, 6, e12373.

39.    Kai Li, Jia-Wei Zhang, Feng Li, Xin-Qing Zhao, Feng-Wu Bai, Hao Song*, Chen-Guang Liu*, Electrochemical Control of Cell Metabolism Improves Ethanol Production of Zymomonas mobilis in an Electro-Fermentation System, ACS Sustainable Chemistry & Engineering, 2023, 11 (6): 2364-2374.

40.    Congcong Huang^#, Yaru Chen^#, Shuai Cheng^#, Mengxu Li, Luxin Wang, Meijie Cheng, Feng Li, Yingxiu Cao* and Hao Song*, Enhanced acetate utilization for value-added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis, Biotechnology and Bioengineering, 2023, 120 (10): 3013-3024.

41.    Peng Long; Mengmeng Qin; Baocai Zhang; Qijing Liu; Fulai Zhao; Zijie Wu; Zhengyang Ni; Huitao Yu; Feng Li; Hao Song; Yiyu Feng*; Wei Feng*, Nano-flower like CoFe-layered double hydroxide@reduced graphene oxide with efficient oxygen reduction reaction for high-power air-cathode microbial fuel cells, Carbon, 2023, 212 (5):118088.

42.    Zijie Wu, Mengmeng Qin, Baocai Zhang, Qijing Liu, Fulai Zhao, Peng Long, Zhengyang Ni, Feng Li, Huitao Yu, Hao Song, Yiyu Feng* and Wei Feng*, High-Power Microbial-Fuel-based Hybrid Cells with Three-Dimensional Graphene-coated Iron Foam Anode by Controlling Fe³⁺ Release, SmartMat (accepted)

43.    Nan Yang, Yao Tian, Mai Zhang, Feng Li, Xiting Peng, Jianxun Li, Yi Li, Bei Fan, Fengzhong Wang*, and Hao Song*, Photocatalyst-Enzyme Hybrid Systems for Light-Driven Biotransformation”, Biotechnology Advances, 2022, 54: 107808.

44.    Bo‐Yu Geng, Lian‐Ying Cao, Feng Li, Hao Song, Chen‐Guang Liu*, Xin‐Qing Zhao and Feng‐Wu Bai, Potential of Zymomonas mobilis as an electricity producer in ethanol production, Biotechnology for Biofuels, 2020, 13: 36.

45.    Ziyin Zhang, Feng Li, Yingxiu Cao, Hong Sun, Yao Tian, Zhenpeng Yu, Guoping Wang, Yiqun Li, Xiaoqiong Ye, Hao Song*, Electricity-driven P450 Enzyme-catalyzed 7α-hydroxylation of steroid in engineered yeast, Catalysis Science & Technology, 2019,9,4877–4887. (Journal Front Cover)

46.    Jialin Meng#, Yao Tian#, Xiaohui Wu, Yinuo Zhou, Jiansheng Li, Nan Yang, Yongchao Zong, Feng Li, Yingxiu Cao, and Hao Song*, “Thiophene-Modified Doubleshell Hollow g-C3N4 Nanosphere Boosts NADH Regeneration via Synergistic Enhancement of Charge Excitation and Separation”, Catalysis Science & Technology, 2019, 9, 1911 - 1921.

47.    Shutian Kong, Juntao Zhao, Feng Li, Tao Chen, Zhiwen Wang*. Advances in anode materials for microbial fuel cells, Energy Technology, 2022, 2200824.

48.    Yingxiu Cao＃, Xiaofei Li＃, Feng Li, Hao Song*, “CRISPRi-sRNA: transcriptional-translational regulation of extracellular electron transfer of Shewanella oneidensis, ACS Synthetic Biology, DOI: 10.1021/acssynbio.6b00374, 2017 in press.

49.    Ying-Xiu Cao, Meng-yuan Song, Feng Li, Congfa Li, Xue Lin, Hao Song*, Development of Synthetic Gene Toolkit of Shewanella oneidensis MR-1, Frontiers in Microbiology, 2019,10:410.

50.    Yefu Chen*, Jian Guo, Feng Li, Mingming Liu, Xinxin Zhang, Xuewu Guo, Dongguang Xiao. Production of pullulan from xylose and hemicelluloses hydrolysate by Aureobasidium pullulans AY82 with pH control and DL-dithiothreitol addition. Biotechnology and Bioprocess Engineering. 2014, 19(2): 282-288.

51.    Deguang Wu, Xuewu Guo, Jun Lu, Xi Sun, Feng Li, Yefu Chen, Dongguang Xiao. A rapid and efficient one-step site-directed deletion, insertion, and substitution mutagenesis protocol. Analytical Biochemistry. 2013,434(2):254-8

52.    张保财#，王忆芸#，石思程，李锋，宋浩*，人工电活性微生物菌群的设计与应用，《生物工程学报》, 2023, 39 (3): 858-880.

53.    宋浩*，贾继朝，张保财，李锋，邵舒琳，微生物燃料电池堆栈的设计开发与应用进展，《天津大学学报（自然科学与工程技术版）》, 2023, 56, 9：887-902.

54.    丁倩，曹英秀，李锋，林童，陈媛媛，陈正，宋浩*，共轭聚合物-产电菌”复合生物电极构建及其在微生物燃料电池中的应用，《生物工程学报》, 2021, 37(1): 1−14.

55.    黄亦馨，李晓，赵津莹，李锋，宋浩*，骆健美*，一株耐盐产电菌Shewanella algae E-1的分离及其产电特性分析，《微生物通报》，2020, 47(2): 351−361。

       

       三、申请专利：

1.       一种高产中链脂肪酸酯的酿酒酵母工程菌及其构建方法，中国, 2013-10-03, Z201310375827.6.

2.       希瓦氏菌中木糖和葡萄糖共利用代谢的构建方法，2023-11-07，中国，CN201911096689.1

3.       希瓦氏菌中木糖利用代谢的构建方法，2023-06-27，中国，CN202210023317.1

4.       一种高效定向电子通量至电子转移链的重组希瓦氏菌株的构建方法及用途， 2022-8-26, 中国, 202211032239.8.

5.       一种促进电子传递载体吩嗪-1-羧酸跨膜外排重组希瓦氏菌构建方法与用途， 2022-11-21, 中国, 2022114793681.

6.       一种通过表达异源孔蛋白增强重组希瓦氏菌细胞通透性的方法及用途，2022-11-21, 中国, 2022114795704.

7.       一种利用外膜孔蛋白 Omp35 促进电子传递载体分泌传递的重组希瓦氏菌构建方法及用途， 2022-10-18, 中国, 2022114650495.

8.       一种表达内膜外排泵 PA3718 加速吩嗪-1-羧酸分泌的重组希瓦氏菌构建方法及用途， 2022-10-17, 中国, 2022114360100.

9.       一种表达外膜孔蛋白 OmpPst1 以加速电子传递速率的重组希瓦氏菌构建方法， 2022-10-17, 中国, 2022114706202.

10.    一种合成与分泌高效电子传递载体吩嗦-1-羚酸的重组希瓦氏菌株构建方法及用途， 2022-10-16, 中国, 2022112787760.

11.    一种产核黄素的重组海藻希瓦氏菌及在产电中的应用，2020-5-29,中国,CN202010478390.9.

12.    一株海藻希瓦氏菌scs-1及其在微生物产电中的应用，2020-5-29,中国,CN202010478446.0.

13.    产电重组希瓦氏菌株及构建方法及用途，2019-9-29,中国,CN201910930577.5.

14.    产电希瓦氏重组菌株及构建方法及用途，2019-9-29,中国,CN201910930539.X.

15.    产电希瓦氏工程菌株及构建方法及用途，2019-9-29,中国，CN201910930372.7.

16.    一种产电重组希瓦氏菌株及构建方法及用途，2019-9-29,中国,CN201910930651.3.

17.    一种抑制细胞分裂提高希瓦氏菌电子传递能力的方法.202211143415.5

18.    一种工程强化希瓦氏菌囊泡分泌提高电能输出的方法.202211105774.1