王智文

现任职称/职务：特聘研究员（英才副教授、博士生导师）
通讯地址：天津大学北洋园校区54E256
电子邮箱：zww@tju.edu.cn
办公电话：qq:2845245149

论著专利:

论文：

54. Shutian Kong, Juntao Zhao, Jiahao Luo, Qianqian Yuan, Tao Chen, Hongwu Ma, Zhiwen Wang*. Co-production of bioelectricity and acetoin by unbalanced fermentation of glycerol in Shewanella oneidensis based on a genome-scale metabolic network   , ACS Sustainable Chemistry & Engineering, 2023, 11, 11890-11899.

53. Shiqi Luo, Juntao Zhao, Yangyang Zheng, Tao Chen, Zhiwen Wang*.    Biosynthesis of nicotinamide mononucleotide: current meta-bolic engineering strategies, challenges, and prospects, Fermentation, 2023, 9, 594.

52. Jian Wu, Meiru Jiang, Shutian Kong, Kunqiang Hong, Juntao Zhao, Xi Sun, Zhenzhen Cui, Tao Chen, Zhiwen Wang*. Unveiling the effect of NCgl0580 gene deletion on 5-aminolevulinic acid biosynthesis in Corynebacterium glutamicum , Fermentation, 2023, 9, 123.

51. Juntao Zhao, Feng Li, Shutian Kong, Tao Chen, Hao Song, Zhiwen Wang*. Elongated riboflavin-producing Shewanella oneidensis in a hybrid biofilm boosts extracellular electron transfer. Advanced Science, 2023, 2206622.

50. Xin Song, Yangyang Zheng, Shuting Li, Alaksh Choudhury, Xunli Liu, Tao Chen, Ryan T Gill, Zhiwen Wang*. Engineering global regulators for enhanced tolerance to multiple inhibitors by CREATE, AIChE Journal, 2023, e18031.

49. Ke Wu, Zhitao Mao, Yufeng Mao, Jinhui Niu, Jingyi Cai, Qianqian Yuan, Lili Yun, Xiaoping Liao, Zhiwen Wang*, Hongwu Ma*. ecBSU1: a genome-scale enzyme-constrained model of Bacillus subtilis based on the ECMpy workflow, Microorganisms, 2023, 11, 178.

48. Xi Sun, Yufeng Mao, Jiahao Luo, Pi Liu, Meiru Jiang, Guimei He, Zhidan Zhang, Qichen Cao, Jie Shen, Hongwu Ma, Tao Chen, Zhiwen Wang*.    Global cellular metabolic rewiring adapts Corynebacterium glutamicum to efficient nonnatural xylose utilization. Applied and Environmental Microbiology, 2022, 88(23): e01518-22.

47. Shutian Kong, Juntao Zhao, Feng Li, Tao Chen, Zhiwen Wang*. Advances in anode materials for microbial fuel cells, Energy Technology, 2022, 2200824.

46. Guimei He, Meiru Jiang, Zhenzhen Cui, Xi Sun, Tao Chen, Zhiwen Wang*. Construction of 5-aminolevulinic acid synthase variants by cysteine-targeted mutation to release heme inhibition, Journal of Bioscience and Bioengineering, 2022, 134(5): 416-423.

45. Jiahao Luo, Qianqian Yuan, Yufeng Mao, Fan Wei, Juntao Zhao, Wentong Yu, Shutian Kong, Yanmei Guo, Xiaoping Liao, Zhiwen Wang*, Hongwu Ma*. Reconstruction of a genome-scale metabolic network for Shewanella oneidensis MR-1 and analysis of its metabolic potential for bioelectrochemical systems , Frontiers in Bioengineering and Biotechnology, 2022, 10: 913077.

44. Meiru Jiang, Kunqiang Hong, Yufeng Mao, Hongwu Ma, Tao Chen, Zhiwen Wang*. Natural 5-aminolevulinic acid: sources, biosynthesis, detection and applications. Frontiers in Bioengineering and Biotechnology, 2022, 10: 841443.

43. Yangyang Zheng, Shutian Kong, Shiqi Luo, Cong Chen, Zhenzhen Cui, Xi Sun, Tao Chen, Zhiwen Wang*. Improving furfural tolerance of Escherichia coli by integrating adaptive laboratory evolution with CRISPR-enabled trackable genome engineering (CREATE), ACS Sustainable Chemistry & Engineering. 2022, 10(7): 2318-2330.

42. Zhenzhen Cui#, Zhiwen Wang#, Meiyu Zheng , Tao Chen*. Advances in biological production of acetoin: a comprehensive overview. Critical Reviews in Biotechnology, 2022, 42(8): 1135-1156.

41. Yangyang Zheng, Kunqiang Hong, Baowei Wang, Dingyu Liu, Tao Chen, and Zhiwen Wang*. Genetic diversity for accelerating microbial adaptive laboratory evolution. ACS Synthetic Biology, 2021, 10, 7, 1574-1586.

40. Cong Chen, Zhenzhen Cui, Juntao Zhao, Shuting Li, Xiaoting Ren, Tao Chen, Zhiwen Wang *. Improving diacetyl production in Corynebacterium glutamicum via modifying respiratory chain. Journal of Biotechnology, 2021, 332: 20-28.

39. Cong Chen, Tao Chen, Zhiwen Wang*, Xueming Zhao. A comparative analysis of China and other countries in metabolic engineering: output, impact and collaboration. Chinese Journal of Chemical Engineering, 2021, 30: 37-45.

38. Juntao Zhao, Feng Li, Yingxiu Cao, Tao Chen, Hao Song, Zhiwen Wang*. Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms, Biotechnology Advances, 2020, https://doi.org/10.1016/j.biotechadv.2020.107682.

37. Zhenzhen Cui,‡  Yufeng Mao,‡  Yujiao Zhao, Meiyu Zheng,  Zhiwen Wang,* Hongwu M *, Tao Chen*. One-pot efficient biosynthesis of (3R)-acetoin from pyruvate by a two-enzyme cascade, Catalysis Science & Technology, 2020, 10: 7734-7744 

36. Cong Chen, Alaksh Choudhury, Shuanghong Zhang, Andrew D. Garst, Xin Song, Xunli Liu, Tao Chen, Ryan T. Gill, Zhiwen Wang*. Integrating CRISPR-Enabled Trackable Genome Engineering and Transcriptomic Analysis of Global Regulators for Antibiotic Resistance Selection and Identification in Escherichia coli, mSystems, 2020, 5(2): e00232-20. 

35. Kun-Qiang Hong, Xiao-Meng Fu, Hao Yin, Shu-Ting Li, Tao Chen, Zhi-Wen Wang*. Advances in the Extraction, Purification and Detection of the Natural Product 1-Deoxynojirimycin, Critical Reviews in Analytical Chemistry, 2020, 1-12.

34. Dingyu Liu, Can Huang, Jiaxin Guo, Peiji Zhang, Tao Chen, Zhiwen Wang*, Xueming Zhao. Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis, Biotechnology for Biofuels, 2019, 12:197.

33.  Zhenzhen Cui, Yujiao Zhao, Yufeng Mao, Ting Shi, Lingxue Lu, Hongwu Ma, Zhiwen Wang*, Tao Chen.     In vitro biosynthesis of optically pure d‐(−)‐acetoin from meso‐2,3‐butanediol using 2,3‐butanediol dehydrogenase and NADH oxidase, Journal of Chemical Technology & Biotechnology, 2019, 94: 2547–2554.

32. Guanglu Wang, Ting Shi, Tao Chen, Xiaoyue Wang, Yongcheng Wang, Dingyu Liu, Jiaxin Guo, Jing Fu, Lili Feng, ZhiwenWang*, Xueming Zhao. Integrated whole-genome and transcriptome sequence analysis reveals the genetic characteristics of a riboflavin-overproducing Bacillus subtilis. Metabolic Engineering, 2018, 48: 138-149.

31. Dingyu Liu, Zhitao Mao, Jiaxin Guo, Leyi Wei, Hongwu Ma, Yajie Tang, Tao Chen, Zhiwen Wang*, Xueming Zhao. Construction, model-based analysis, and characterization of a promoter library for fine-tuned gene expression in Bacillus subtilis. ACS Synthetic Biology, 2018, 7: 1785-1797.

30. Yufeng Mao, Guiying L, Zhishuai Chan, Ran Tao, Zhenzhen Cui, Zhiwen Wang*, Yajie Tang, Tao Chen*, Xueming Zhao. Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate.     Biotechnology for Biofuels, 2018, 11(1) :95.

29. Zhenzhen Cui, Yufeng Mao, Yujiao Zhao, Cong Chen, Yajie Tang, Tao Chen, Hongwu Ma*, Zhiwen Wang*. Concomitant cell-free biosynthesis of optically pure D-(-)-acetoin and xylitol via a novel NAD+ regeneration in two-enzyme cascade. Journal of Chemical Technology & Biotechnology, 2018, 93: 3444-3451.

28. Kunqiang Hong, Dingyu Liu, Tao Chen, Zhiwen Wang*. Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis. World Journal of Microbiology and Biotechnology, 2018, 34:153.

26. Feiran Li, Wei Xie, Qianqian Yuan, Hao Luo, Peishun Li, Tao Chen, Xueming Zhao, Zhiwen Wang*, Hongwu Ma*. Genome-scale metabolic model analysis indicates low energy production efficiency in marine ammonia-oxidizing archaea. AMB Express, 2018, 8: 106.

25. Shuanghong Zhang, Dingyu Liu, Zhitao Mao, Yufeng Mao, Hongwu Ma, Tao Chen, Xueming Zhao, Zhiwen Wang*. Model-based reconstruction of synthetic promoter library in Corynebacterium glutamicum. Biotechnology Letters,    2018, 40(5): 819-827.

24. Yalan Zou, Tao Chen, Lili Feng, Shuanghong Zhang, Dongxu Xing, Zhiwen Wang*. Enhancement of 5-aminolevulinic acid production by metabolic engineering of the glycine biosynthesis pathway in Corynebacterium glutamicum. Biotechnology Letters, 2017,39 (9),1369-1374.

23. Qianqian Yuan, Teng Huang, Peishun Li, Tong Hao, Feiran Li, Hongwu Ma*, Zhiwen Wang*, Xueming Zhao, Tao Chen, Igor Goryanin. Pathway-Consensus Approach to Metabolic Network Reconstruction for Pseudomonas putida KT2440 by Systematic Comparison of Published Models. PLoS One. 2017, 12(1):e0169437

22. Yufeng Mao, Jing Fu, Ran Tao, Can Huang, Zhiwen Wang*, Yajie Tang, Tao Chen*, Xueming Zhao. Systematic metabolic engineering of Corynebacterium glutamicum for industrial-level production of optically pure D-(-)-Acetoin. Green Chemistry. 2017,19, 5691-5702.

21. Garst AD, Bassalo MC1, Pines G, Lynch SA, Halweg-Edwards AL, Liu R, Liang L, Wang Z, Zeitoun R, Alexander WG, Gill RT. Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering        Nature Biotechnology. 2017, 35(1):48-55.

20. Bo Zhang, Xin-li Li, Jing Fu, Ning Li, Zhiwen Wang*, Ya-jie Tang,Tao Chen. Production of Acetoin through Simultaneous Utilization of Glucose, Xylose, and Arabinose by Engineered Bacillus subtilis, PLoS One. 2016,11(7): e0159298.

19. Jiao Meng, Baiyun Wang, Dingyu Liu, Tao Chen, Zhiwen Wang, Xueming Zhao. High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli. Microbial Cell Factories. 2016,15: 141.

18. Xiaoyue Wang, Guanglu Wang, Xinli Li, Jing Fu, Tao Chen, Zhiwen Wang*, Xueming Zhao. Directed evolution of adenylosuccinate synthetase from Bacillus subtilis and its application in metabolic engineering. Journal of Biotechnology, 2016,231:115-121.

17. Jing Fu, Guangxin Huo, Lili Feng, Yufeng Mao, Zhiwen Wang*, Hongwu Ma, Tao Chen*, Xueming Zhao. Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production. Biotechnology for Biofuels, 2016,9:90

16. Lili Feng,Ya Zhang, Jing Fu,Yufeng Mao, Tao Chen, Xueming Zhao, Zhiwen Wang*. Metabolic engineering of Corynebacterium glutamicum for efficient production of 5-aminolevulinic acid. Biotechnology and Bioengineering, 2016,113:1284–1293.

15. Wenwen Ma, Xiaoyue Wang, Yufeng Mao, Zhiwen Wang*, Tao Chen, Xueming Zhao. Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker. Biotechnology Letters, 2015,37(3):609-617.

14. Xin-Bo ZHANG, Xiang-Ming MA, Bai-Yun WANG, Xiang-Hui MA, Zhi-Wen WANG*. Selective pressure on synonymous codon usage in mammalian protein-encoding genes. Journal of Systematics and Evolution, 2015,53 (2): 191-195

Wang Xiaoyue, Wang Baiyun, Wang Zhiwen*, Chen Tao, Zhao Xueming. The research progress of protein directed evolution. Progress in Biochemistry and Biophysics2015, 42(2): 123-131

13. Yifan Li, ZhenquanLin, Can Huang, Yan Zhang, Zhiwen Wang, Ya-jieTang, TaoChen, Xueming Zhao. Metabolic engineeringof Escherichiacoli using CRISPR–Cas9 meditated genome editing . Metabolic engineering. 2015,31:13-21.

12. Jing Fu, Zhiwen Wang(Co-first author), Tao Chen*, et al.. NADH Plays the vital role for chiral pure D-(-)-2,3-butanediol production in Bacillus subtilis under limited oxygen, Biotechnology and Bioengineering, 2014, Doi: 10.1002/bit.25265.

11. Ting Shi, Yongcheng Wang, Zhiwen Wang*, Guanglu Wang, Dingyu Liu, Jing Fu, Tao Chen, Xueming Zhao. Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis, Microbial Cell Factories, 2014,13:101.  

10. Zhenquan Lin,Zhibo Xu,Yifan Li, Zhiwen Wang*,Tao Chen*, Xueming Zhao. Metabolic engineering of Escherichia coli for the production of riboflavin,  Microbial Cell Factories, 2014,13:104.

9. Xianghui Ma, Xinbo Zhang, Baiyun Wang, Yufeng Mao, Zhiwen Wang*,Tao Chen*, Xueming Zhao. Engineering microorganisms based on molecular evolutionary analysis: a succinate production case study, Evolutionary Applications, 2014,doi:10.1111/eva.12186.

8. Guanglu Wang, Ling Bai, Zhiwen Wang*, Ting Shi, Tao Chen, Xueming Zhao. Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis, World J Microbiol Biotechnol, 2014, 30:1893–1900.

7. Yang, J., Z. Wang(Co-first author), N. Zhu, B. Wang, T. Chen*, X. Zhao. Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate, Microbiological Research, 2014, 169:432-40.

6.  Wenwen Ma, Xiaoyue Wang, Yufeng Mao, Zhiwen Wang*, Tao Chen, Xueming Zhao. Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker, Biotechnology Letters, 2014,DOI: 10.1007/s10529-014-1718-8.

5. Ting Shi, Guanglu Wang, Zhiwen Wang*, et al.. Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromosome, PloS One, 2013, 8(11):e81370.

3. Zhiwen Wang, Tao Chen*, Xianghui Ma, et al.. Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum, Bioresource Technology, 2011, 102(4): 3934-3940.

2. Xianghui Ma*, Zhiwen Wang. Anticancer drug discovery in the future: an evolutionary perspective, Drug Discovery Today, 2009, 14(23):1136-1142.

1. Zhiwen Wang, Xunli Liu*. Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp. using response surface methodology, Bioresource Technology, 2008, 99(17):8245-8251.

专著：

王智文, 陈涛，赵学明. 《代谢工程与合成生物学》第二版，2016.06.

赵学明，陈涛，王智文等. 代谢工程，高等教育出版社，2015.03.

中文论文：

13. 李书廷, 洪坤强, 汪保卫, 陈聪, 陈涛, 王智文*.  大肠杆菌乙酸耐受性菌株的构建及其耐受机制研究进展. 微生物学通报, 2020, 47(12): 4250−4259.

12. 郭佳欣，张培基，刘丁玉，洪坤强，陈涛，王智文*．常压室温等离子体诱变选育高产核黄素枯草芽孢杆菌. 食品与发酵工业, 2020, 46(4): 28-33．

11. 崔真真, 毛雨丰, 陈聪, 袁倩倩, 王智文*, 陈 涛. 生物法合成双乙酰的研究进展. 生物加工过程. 2017,15(5):57-64.

10. 张双虹, 邹亚兰,宋鑫, 冯丽丽, 陈 涛, 王智文*. 代谢工程合成 5 氨基乙酰丙酸的研究进展.生物加工过程. 2017,15(5):65-70.

9. 刘丁玉,孟娇,王智文*,陈涛,赵学明. 多元模块工程在代谢工程中的应用与研究进展. 化工进展. 2016,35(11):3619-3626.

8. 余君涵, 马雯雯,王智文*, 陈涛, 赵学明. 人工合成启动子文库研究进展. 微生物学通报. 2016,43(1):198−204.

7. 王永成, 陈涛, 石婷, 王智文*, 赵学明. 嘌呤核苷及其衍生物的代谢工程. 中国生物工程杂志. 2015,35(5):87-95

6. 李桂莹, 张新波, 王智文*, 石婷，陈涛, 赵学明. 逆向代谢工程的最新研究进展. 生物工程学报. 2014, 30(8): 1−13.

5. 王智文, 马向辉, 陈洵，赵学明，陈涛*. 大肠杆菌生产CoQ10 的代谢工程研究进展与工程策略. 化工进展. 2009, 28(5):855-864

4. 王智文，袁士涛，何亮，等. 多粘类芽孢杆菌Cp-S316抗真菌活性物质的提取及其部分性质研究. 农业环境科学学报. 2007,26(4):1464-1468

3. 王智文，刘训理*，何亮，等. Cp-S316菌株发酵培养基的优化及其对烟草赤星病菌的抑制作用. 农业环境科学学报. 2007,26(2):723-728

2. 王智文，刘训理*. 芽孢杆菌非核糖体肽的研究进展. 蚕业科学. 2006,32(3):357-361

1. 刘训理*，王智文，孙海新，等. 圆孢芽孢杆菌A95抗菌蛋白的分离纯化与性质研究. 蚕业科学. 2006,32(3):392-398

专利：

11. 王智文，崔真真，毛雨丰，马红武，赵玉娇，陈涛. 一种体外酶反应生产L(+)-乙偶姻的方法. 201710215961.8

10. 王智文，毛雨丰，常志帅，陈涛，赵学明. 利用秸秆水解液高产琥珀酸的谷氨酸帮杆菌及构建及应用. 201710215458.2

9. 王智文，毛雨丰，陈涛，赵学明. 高产琥珀酸的谷氨酸棒状杆菌菌株构建方法及应用. 201710215454.4

8. 王智文, 邹亚兰, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌工程菌株构建. 2016109882995

7. 王智文, 邹亚兰, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌重组菌株构建. 2016109868377

6. 王智文, 冯丽丽, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌工程菌株构建. 201610888618.5

5. 王智文, 冯丽丽, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌菌株构建及应用. 2016104086744

4. 王智文，王光璐，石婷，陈涛，赵学明. 枯草芽胞杆菌基因组无痕修饰方法, 201310375944.2.

3. 王智文，王光璐，石婷，陈涛，赵学明. 枯草芽孢杆菌腺苷琥珀酸合成酶突变基因purA的序列及应用, 201410150346.X.

2. 王智文，王光璐，石婷，陈涛，赵学明. 枯草芽孢杆菌双组分调控系统突变基因yvrH及应用, 201410150235.9.

1. 王智文，石婷，王永成，王光璐，陈涛，赵学明. 枯草芽孢杆菌编码PRPP转酰胺酶突变基因purF及应用, 201410150391.5.