34. Dingyu Liu, Can Huang, Jiaxin Guo, Peiji Zhang, Tao Chen, Zhiwen Wang*, Xueming Zhao. Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis, Biotechnology for Biofuels, 2019, 12:197.

33.  Zhenzhen Cui, Yujiao Zhao, Yufeng Mao, Ting Shi, Lingxue Lu, Hongwu Ma, Zhiwen Wang*, Tao Chen.     In vitro biosynthesis of optically pure d‐(−)‐acetoin from meso‐2,3‐butanediol using 2,3‐butanediol dehydrogenase and NADH oxidase, Journal of Chemical Technology & Biotechnology, 2019, 94: 2547–2554.

32. Guanglu Wang, Ting Shi, Tao Chen, Xiaoyue Wang, Yongcheng Wang, Dingyu Liu, Jiaxin Guo, Jing Fu, Lili Feng, ZhiwenWang*, Xueming Zhao. Integrated whole-genome and transcriptome sequence analysis reveals the genetic characteristics of a riboflavin-overproducing Bacillus subtilis. Metabolic Engineering, 2018, 48: 138-149.

31. Dingyu Liu, Zhitao Mao, Jiaxin Guo, Leyi Wei, Hongwu Ma, Yajie Tang, Tao Chen, Zhiwen Wang*, Xueming Zhao. Construction, model-based analysis, and characterization of a promoter library for fine-tuned gene expression in Bacillus subtilis. ACS Synthetic Biology, 2018, 7: 1785-1797.

30. Yufeng Mao, Guiying L, Zhishuai Chan, Ran Tao, Zhenzhen Cui, Zhiwen Wang*, Yajie Tang, Tao Chen*, Xueming Zhao. Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate.     Biotechnology for Biofuels, 2018, 11(1) :95.

29. Zhenzhen Cui, Yufeng Mao, Yujiao Zhao, Cong Chen, Yajie Tang, Tao Chen, Hongwu Ma*, Zhiwen Wang*. Concomitant cell-free biosynthesis of optically pure D-(-)-acetoin and xylitol via a novel NAD+ regeneration in two-enzyme cascade. Journal of Chemical Technology & Biotechnology, 2018, 93: 3444-3451.

28. Kunqiang Hong, Dingyu Liu, Tao Chen, Zhiwen Wang*. Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis. World Journal of Microbiology and Biotechnology, 2018, 34:153.

26. Feiran Li, Wei Xie, Qianqian Yuan, Hao Luo, Peishun Li, Tao Chen, Xueming Zhao, Zhiwen Wang*, Hongwu Ma*. Genome-scale metabolic model analysis indicates low energy production efficiency in marine ammonia-oxidizing archaea. AMB Express, 2018, 8: 106.

25. Shuanghong Zhang, Dingyu Liu, Zhitao Mao, Yufeng Mao, Hongwu Ma, Tao Chen, Xueming Zhao, Zhiwen Wang*. Model-based reconstruction of synthetic promoter library in Corynebacterium glutamicum. Biotechnology Letters,    2018, 40(5): 819-827.

24. Yalan Zou, Tao Chen, Lili Feng, Shuanghong Zhang, Dongxu Xing, Zhiwen Wang*. Enhancement of 5-aminolevulinic acid production by metabolic engineering of the glycine biosynthesis pathway in Corynebacterium glutamicum. Biotechnology Letters, 2017,39 (9),1369-1374.

23. Qianqian Yuan, Teng Huang, Peishun Li, Tong Hao, Feiran Li, Hongwu Ma*, Zhiwen Wang*, Xueming Zhao, Tao Chen, Igor Goryanin. Pathway-Consensus Approach to Metabolic Network Reconstruction for Pseudomonas putida KT2440 by Systematic Comparison of Published Models. PLoS One. 2017, 12(1):e0169437

22. Yufeng Mao, Jing Fu, Ran Tao, Can Huang, Zhiwen Wang*, Yajie Tang, Tao Chen*, Xueming Zhao. Systematic metabolic engineering of Corynebacterium glutamicum for industrial-level production of optically pure D-(-)-Acetoin. Green Chemistry. 2017,19, 5691-5702.

21. Garst AD, Bassalo MC1, Pines G, Lynch SA, Halweg-Edwards AL, Liu R, Liang L, Wang Z, Zeitoun R, Alexander WG, Gill RT. Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering        Nature Biotechnology. 2017, 35(1):48-55.

20. Bo Zhang, Xin-li Li, Jing Fu, Ning Li, Zhiwen Wang*, Ya-jie Tang,Tao Chen. Production of Acetoin through Simultaneous Utilization of Glucose, Xylose, and Arabinose by Engineered Bacillus subtilis, PLoS One. 2016,11(7): e0159298.

19. Jiao Meng, Baiyun Wang, Dingyu Liu, Tao Chen, Zhiwen Wang, Xueming Zhao. High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli. Microbial Cell Factories. 2016,15: 141.

18. Xiaoyue Wang, Guanglu Wang, Xinli Li, Jing Fu, Tao Chen, Zhiwen Wang*, Xueming Zhao. Directed evolution of adenylosuccinate synthetase from Bacillus subtilis and its application in metabolic engineering. Journal of Biotechnology, 2016,231:115-121.

17. Jing Fu, Guangxin Huo, Lili Feng, Yufeng Mao, Zhiwen Wang*, Hongwu Ma, Tao Chen*, Xueming Zhao. Metabolic engineering of Bacillus subtilis for chiral pure meso-2,3-butanediol production. Biotechnology for Biofuels, 2016,9:90

16. Lili Feng,Ya Zhang, Jing Fu,Yufeng Mao, Tao Chen, Xueming Zhao, Zhiwen Wang*. Metabolic engineering of Corynebacterium glutamicum for efficient production of 5-aminolevulinic acid. Biotechnology and Bioengineering, 2016,113:1284–1293.

15. Wenwen Ma, Xiaoyue Wang, Yufeng Mao, Zhiwen Wang*, Tao Chen, Xueming Zhao. Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker. Biotechnology Letters, 2015,37(3):609-617.

14. Xin-Bo ZHANG, Xiang-Ming MA, Bai-Yun WANG, Xiang-Hui MA, Zhi-Wen WANG*. Selective pressure on synonymous codon usage in mammalian protein-encoding genes. Journal of Systematics and Evolution, 2015,53 (2): 191-195

Wang Xiaoyue, Wang Baiyun, Wang Zhiwen*, Chen Tao, Zhao Xueming. The research progress of protein directed evolution. Progress in Biochemistry and Biophysics2015, 42(2): 123-131

13. Yifan Li, ZhenquanLin, Can Huang, Yan Zhang, Zhiwen Wang, Ya-jieTang, TaoChen, Xueming Zhao. Metabolic engineeringof Escherichiacoli using CRISPR–Cas9 meditated genome editing . Metabolic engineering. 2015,31:13-21.

12. Jing Fu, Zhiwen Wang(Co-first author), Tao Chen*, et al.. NADH Plays the vital role for chiral pure D-(-)-2,3-butanediol production in Bacillus subtilis under limited oxygen, Biotechnology and Bioengineering, 2014, Doi: 10.1002/bit.25265.

11. Ting Shi, Yongcheng Wang, Zhiwen Wang*, Guanglu Wang, Dingyu Liu, Jing Fu, Tao Chen, Xueming Zhao. Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis, Microbial Cell Factories, 2014,13:101.  

10. Zhenquan Lin,Zhibo Xu,Yifan Li, Zhiwen Wang*,Tao Chen*, Xueming Zhao. Metabolic engineering of Escherichia coli for the production of riboflavin,  Microbial Cell Factories, 2014,13:104.

9. Xianghui Ma, Xinbo Zhang, Baiyun Wang, Yufeng Mao, Zhiwen Wang*,Tao Chen*, Xueming Zhao. Engineering microorganisms based on molecular evolutionary analysis: a succinate production case study, Evolutionary Applications, 2014,doi:10.1111/eva.12186.

8. Guanglu Wang, Ling Bai, Zhiwen Wang*, Ting Shi, Tao Chen, Xueming Zhao. Enhancement of riboflavin production by deregulating gluconeogenesis in Bacillus subtilis, World J Microbiol Biotechnol, 2014, 30:1893–1900.

7. Yang, J., Z. Wang(Co-first author), N. Zhu, B. Wang, T. Chen*, X. Zhao. Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate, Microbiological Research, 2014, 169:432-40.

6.  Wenwen Ma, Xiaoyue Wang, Yufeng Mao, Zhiwen Wang*, Tao Chen, Xueming Zhao. Development of a markerless gene replacement system in Corynebacterium glutamicum using upp as a counter-selection marker, Biotechnology Letters, 2014,DOI: 10.1007/s10529-014-1718-8.

5. Ting Shi, Guanglu Wang, Zhiwen Wang*, et al.. Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromosome, PloS One, 2013, 8(11):e81370.

3. Zhiwen Wang, Tao Chen*, Xianghui Ma, et al.. Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum, Bioresource Technology, 2011, 102(4): 3934-3940.

2. Xianghui Ma*, Zhiwen Wang. Anticancer drug discovery in the future: an evolutionary perspective, Drug Discovery Today, 2009, 14(23):1136-1142.

1. Zhiwen Wang, Xunli Liu*. Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp. using response surface methodology, Bioresource Technology, 2008, 99(17):8245-8251.


赵学明,陈涛,王智文等. 代谢工程,高等教育出版社,2015.03.


11. 崔真真, 毛雨丰, 陈聪, 袁倩倩, 王智文, 陈 涛. 生物法合成双乙酰的研究进展. 生物加工过程. 2017,15(5):57-64.

10. 张双虹, 邹亚兰,宋鑫, 冯丽丽, 陈 涛, 王智文. 代谢工程合成 5 氨基乙酰丙酸的研究进展.生物加工过程. 2017,15(5):65-70.

9. 刘丁玉,孟娇,王智文,陈涛,赵学明. 多元模块工程在代谢工程中的应用与研究进展. 化工进展. 2016,35(11):3619-3626.

8. 余君涵, 马雯雯,王智文, 陈涛, 赵学明. 人工合成启动子文库研究进展. 微生物学通报. 2016,43(1):198−204.

7. 王永成陈涛石婷王智文赵学明. 嘌呤核苷及其衍生物的代谢工程. 中国生物工程杂志. 2015,35(5):87-95

6. 李桂莹, 张新波, 王智文*, 石婷,陈涛, 赵学明. 逆向代谢工程的最新研究进展. 生物工程学报. 2014, 30(8): 1−13.

5. 王智文, 马向辉, 陈洵,赵学明,陈涛*. 大肠杆菌生产CoQ10 的代谢工程研究进展与工程策略. 化工进展. 2009, 28(5):855-864

4. 王智文,袁士涛,何亮,等. 多粘类芽孢杆菌Cp-S316抗真菌活性物质的提取及其部分性质研究. 农业环境科学学报. 2007,26(4):1464-1468

3. 王智文,刘训理*,何亮,等. Cp-S316菌株发酵培养基的优化及其对烟草赤星病菌的抑制作用. 农业环境科学学报. 2007,26(2):723-728

2. 王智文,刘训理*. 芽孢杆菌非核糖体肽的研究进展. 蚕业科学. 2006,32(3):357-361

1. 刘训理*,王智文,孙海新,等. 圆孢芽孢杆菌A95抗菌蛋白的分离纯化与性质研究. 蚕业科学. 2006,32(3):392-398


11. 王智文,崔真真,毛雨丰,马红武,赵玉娇,陈涛. 一种体外酶反应生产L(+)-乙偶姻的方法. 201710215961.8

10. 王智文,毛雨丰,常志帅,陈涛,赵学明. 利用秸秆水解液高产琥珀酸的谷氨酸帮杆菌及构建及应用. 201710215458.2

9. 王智文,毛雨丰,陈涛,赵学明. 高产琥珀酸的谷氨酸棒状杆菌菌株构建方法及应用. 201710215454.4

8. 王智文, 邹亚兰, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌工程菌株构建. 2016109882995

7. 王智文, 邹亚兰, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌重组菌株构建. 2016109868377

6. 王智文, 冯丽丽, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌工程菌株构建. 201610888618.5

5. 王智文, 冯丽丽, 陈涛, 赵学明. 生产5-氨基乙酰丙酸的谷氨酸棒杆菌菌株构建及应用. 2016104086744

4. 王智文,王光璐,石婷,陈涛,赵学明. 枯草芽胞杆菌基因组无痕修饰方法, 201310375944.2.

3. 王智文,王光璐,石婷,陈涛,赵学明. 枯草芽孢杆菌腺苷琥珀酸合成酶突变基因purA的序列及应用, 201410150346.X.

2. 王智文,王光璐,石婷,陈涛,赵学明. 枯草芽孢杆菌双组分调控系统突变基因yvrH及应用, 201410150235.9.

1. 王智文,石婷,王永成,王光璐,陈涛,赵学明. 枯草芽孢杆菌编码PRPP转酰胺酶突变基因purF及应用, 201410150391.5.